New 'nanopores' technique offers proof-of-concept of earlier, safer tumor detection

Specific detection of Point-Mutation-Position using biological nanopore.

In recent years, a non-invasive biopsy method called liquid biopsy has shown promise as a potential alternative to tissue biopsy, currently the gold standard in cancer detection and diagnosis. A tissue biopsy sample—traditionally collected through a surgical procedure that may require general anesthesia, accompanied by the risk of complications that may occur from any surgery, from pain through to infection and pneumonia—is typically tested for specific genetic variations, also referred to as mutations, which may offer information on a clear optimal treatment for that cancer.

Liquid biopsies, on the other hand, identify the presence of tumor DNA fragments or cells circulating in bodily fluids like blood, urine or saliva—called circulating tumor DNA (ctDNA)—and spares patients from unnecessary harm. Unfortunately, the minute amount of ctDNA in bodily fluids and their short-lived nature remain a challenge for real-life applications.

But biotech researchers at the Tokyo University of Agriculture and Technology (TUAT) have developed a nanopore technique which, in laboratory tests, has shown potential to offer a powerful, quick-and-easy tool for mutation detection.

The findings were published on Aug 9th, 2020, in the peer-reviewed journal Small Methods.

Nanopore measurements, a third-generation genetic sequencing technology, passes a DNA molecule through a nano-scale hole, or 'pore.' As it transits the pore, the DNA nucleotide bases (adenine [A], cytosine [C], guanine [G], or thymine [T]) cause changes in electrical charge that are specific to each of those bases and which can be cataloged, much like passing sand through a series of sifters. Nanopore tech can also sense the translocation, or exchange of genetic material, of short DNA strands via a blocking of the electric current when the pore is open. In both cases, second-generation measurement run times last anywhere from 4-9 days. But nanopore measurements occur in real time.

The rapid and cheap nanopore technique is often used for whole genome sequencing, but its use for ctDNA analysis remains underdeveloped. Nanopore sequencing is skilled in long read-lengths (>10,000-50,000 nt). Sequencing ctDNA (~150 bp) needs earlier stage processing like giving multiple copies of the original ctDNA to stretch targets. While attempts at approaches using nanopore tech for directly ctDNA detection have been made, and are able to recognize the presence or absence of a single genetic mutation, so far, they have been unable to recognize the position of this mutation.

The TUAT method, based on statistical analysis of the length of time it takes for the genetic code to unzip, and of the blocking of the current, allowing both the presence and position of a mutation to be identified. It has so far only been used on short strips of genetic material, or oligonucleotides, not in real-world liquid biopsies.

"This is still at the proof-of-concept stage, but it is exciting not just because it could permit earlier detection," said Ryuji Kawano, one of the two engineers responsible for devising the new method, "but the technique could be used to assess the degree of metastasis [cancer growth] to how well anticancer drugs are working."

The researchers now hope to work with medical institutions to verify and catalog the location of mutations in ctDNA from a many different cancers in order to develop this method as a simple diagnostic method for a wide range of occurrences of the disease.


MIS-ASIA is an online content marketing platform that has a large number of visitors worldwide. It is considered to be the leading IT, mechanical, chemical, and nanomaterial information distributor in the Asia-Pacific region. The MIS-ASIA website provides high-quality articles and news on digital information technology, mechanical technology, nanotechnology, biology and science for scientists, engineers and industry experts, machinery suppliers and buyers, chemical suppliers and laboratories. If you need advertising and posting service, or you need to start sponsorship, please contact us.

Inquery us

  • tags

Our Latest Products

High Purity Graphite Powder Flake Graphite C Powder CAS 7782-42-5

Item No.: Tr-C High purity graphite powder flake graphite is natural crystalline graphite, which looks like fish phosphate, belongs to the hexagonal crystal system, and has good high-temperature resistance, electrical conductivity.Purity: 99…

Chromium Chip Chromium Sheet Chromium Flake CAS 7440-47-3

Item No.: Tr-Cr02 Chrome chip hard and brittle steel gray bright metal with hardness like corundum and lower melting than platinumPurity>99.95%Product name: chromium flake…

Succinate monoglycerides

About Succinate monoglycerides:Milky powder, insoluble in cold water, can be dispersed in hot water and hot grease.TRUNNANO is a trusted global Succinate monoglyceride. Feel free to send an inquiry to...…

0086-0379-64280201 brad@ihpa.net skype whatsapp