Small: a new way to prepare DNA origami nano patterns with preset pixels

DNA origami is a technology to fold a long str (skeleton str) by hundreds of short strs (stapling str) to prepare DNA nanostructures with arbitrary shape. From the structure point of view a DNA origami is like a dot matrix with hundreds of nanometer pixels. By modifying some staple chains arbitrary patterns can be constructed within the scope of the origami. With the advantages of easy synthesis controllable shape DNA origami has broad application prospects in the fields of biosensor biomedicine nano optoelectronics so on. However according to the existing methods the self-assembly process of DNA origami is extremely complex hundreds of DNA strs are completely rom assembled in solution. Even worse once the origami is folded it is difficult to change the pattern. The only way is to reconstruct the whole staple chain library that is to mix the modified staple chain to be replaced with the remaining ordinary staple chain according to the pixel information of the final pattern. As a staple chain library contains hundreds of chains it will take hundreds of steps to complete the reconstruction if it is operated by h which brings a great challenge to the efficient construction of nano patterns hinders the wide application of DNA origami.

Wang Lihua of Shanghai Institute of Applied Physics Chinese Academy of Sciences Liu Huajie Tongji University etc. cooperated. According to the distinct grid characteristics of DNA origami the idea of preset modified pixels (p-bs) in the corresponding pattern grid was proposed that is the pixels of the designed pattern should be positioned in the corresponding grid in advance then connected with an unmodified one Mix with universal staples. Because the preset pixels occupy the grid points first the general pixels can only occupy the remaining grid points so on the one h the pattern assembly process is regulated on the other h the workload is greatly reduced so a variety of nano patterns are obtained efficiently.

in practical operation the general pixel library is a mixture of common unmodified staple chains used for origami the preset modified pixel chain is divided into two parts namely addressing area function area. The addressing region is a 40 base long sequence which aims to hybridize with the skeleton chain of a specific grid point so it determines the position of the pixel chain in the DNA origami; due to the preferential combination with the skeleton chain it can ensure that the pixel chain will not fall behind in the competition with the ordinary staple chain in the folding process so as to prevent the loss of pixels. Functional regions are regions that perform specific functions are determined by the composition of patterns. For example when constructing streptavidin patterns we modify biotin sites in functional regions. By constructing streptavidin patterns with 1-9 sites comparing with the theoretical yield we confirmed the efficiency feasibility of the pixel chain method in pattern preparation. At the same time due to the use of skeleton chain as the only carrier of pattern information we successfully prepared a variety of different patterns in the same reaction environment the proportion of each group of patterns can be controlled by adjusting the modification of skeleton chain. In addition we have successfully used the end of the toehold chain as the end of the skeleton chain to form a dynamic addressing pattern. By adjusting the functional area this method can be applied to the construction of patterns containing different nano units. We assembled gold nanoparticles by introducing a specific viscous end into the functional region constructed a tetramer structure of gold nanoparticles with optical activity on the origami template. One of the gold nanoparticles the other three were located on both sides of the origami. By changing the addressing region of the corresponding pixel chain we can adjust the position of the nanoparticles on the origami so as to construct a tetramer structure with opposite optical activity. Compared with the traditional method this method only needs a few steps to reconstruct a completely different pattern which greatly simplifies the workload.

proposed a new method to construct DNA origami patterns efficiently which not only regulates the assembly dynamic path greatly simplifies the process of constructing multiple DNA origami patterns makes the application of DNA origami in information storage other fields possible. At the same time due to the separation of the process of pattern information writing (skeleton modification) reading (origami folding) it also provides a reference for the application of this method in secure communication It is possible for the application of.

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