Ukadaulo Wamakono Wa Magnetic Grain Amapereka Chiyero Chapamwamba cha RNA, Bwererani, ndi Kuchita Bwino kwa Phunziro la Molecular.
Shanghai, China– [15th July]– Lnjnbio (Malingaliro a kampani Shanghai Lingjun Biotechnology Co., Ltd.), wotsogolera wotsogola wamankhwala apamwamba a kafukufuku wa sayansi ya moyo, ndiwolemekezeka kulengeza kutulutsidwa kwa Magnetic Grains for Pet Cells Total RNA Removal Kit. Gulu lotsogolali lapangidwa kuti lithandizire kudzipatula kwa RNA yapamwamba kwambiri kuchokera kumagulu ambiri anyama., kupereka asayansi mwaluso, odalirika, ndi njira zodzichitira zokha zokha zomwe zimagwirizana ndi njira zachikhalidwe zozikidwa ndi phenol-chloroform.
Revolutionizing RNA M'zigawo ndi Magnetic Grain Technology
The Lnjnbio Magnetic Beads for Animal Tissue Total RNA Removal Package imagwiritsa ntchito makina osefera opangidwa ndi maginito omwe amapangitsa kuti zinthu zisinthe mwachangu., kudzipatula kwa RNA koyera kwambiri ndi nthawi yocheperako. Pogwiritsa ntchito maximized surface chemistry ndi maginito kupatukana, phukusi izi amachotsa kufunika centrifugation, kusefa vacuum, kapena zosungunulira zachilengedwe zovulaza, kuchepetsa kwambiri nthawi yokonza pamene mukugwiritsa ntchito mwayi wokhazikika wa RNA.
(Lnjnbio Magnetic Beads for Animal Tissue Total RNA Extraction Kit)
Zinthu Zachinsinsi & Ubwino wake
Zodabwitsa za RNA Pureness & Bwererani
Mikanda yapadera ya maginito imamangiriza RNA mwapadera ndikuchotsa zowononga monga mapuloteni, genomic DNA, ndi kupewa kwa enzyme.
Kuchuluka kwa A260/A280 nthawi zonse ≥ 1.9, kupanga kuyera koyenera pamapulogalamu otsika ngati NGS ndi qPCR.
Mwamsanga & Mayendedwe Osavuta Ogwiritsa Ntchito
Kuchotsa kwathunthu pakadutsa mphindi 20– 30 mphindi, kusintha kwakukulu pamachitidwe akale.
Palibe centrifugation kapena mzati kusamutsidwa amafuna– kungosakaniza, kumanga, woyera, ndi elute.
Dongosolo logwira ntchito bwino la lysis barriers limatsimikizira kusokonezeka kwathunthu kwa minofu ngakhale pazitsanzo zolimba kapena zokhala ndi lipid.
Zodzichitira-Zokonzekera Ma Lab Apamwamba
Oyenera kwathunthu ndi ma robot oyendetsa madzimadzi (mwachitsanzo, KingFisher, Biomec, Tekani) kuti mutengeke mopanda msoko muzoyenda zokha.
Zoyenera pa kafukufuku wamkulu wa genomic, kafukufuku wa akatswiri, ndi ntchito mafakitale.
( Electropherogram ya Lnjnbio Magnetic Beads)
Mphamvu Yomanga Yamphamvu: Momwemonso 50 µg RNA pa mg wa mbewu, kutengera kuchuluka kwa zolemera zama cell olowera (10– 50 mg).
RNase-Free Guarantee: Zinthu zonse zimayesedwa mwamphamvu kuti zipewe kuwonongeka kwa RNA.
Ukatswiri Akugogomezera
1. Kupambana Kwambiri kwa Magnetic Grain
Mikanda Make-up: Tinthu tating'ono ta silika tokhala ndi maginito tofanana ndi kukula kwake (1– 3 µm) onetsetsani kuti ntchito yomangirira ya RNA yokhazikika.
Mphamvu Yomanga Yamphamvu: Momwemonso 50 µg RNA pa mg wa mikanda, kukwanira miyeso yambiri yama cell olowera (10– 50 mg).
RNase-Free Chitsimikizo: Zigawo zonse zimawunikidwa mwamphamvu kuti asiye kuwonongeka kwa RNA.
2. Makina Owonjezera a Buffer
Lysis Barrier: Imasokoneza mwachangu ma cell ndikukhazikika kwa RNA, ngakhale m'malo olemera a RNase.
Zovala Zochapira: Chotsani bwino zonyansa popanda kuwononga zokolola za RNA.
Elution Barrier: Kupanga kwa Low-EDTA kumapangitsa kugwirizana kwina ndi zoyeserera zapansi panthaka.
3. Kuwongolera Kwabwino Kwambiri
Gulu lililonse limadutsa kuyesa kwa endotoxin, Kuzindikira kwa DNase/RNase, ndi kulinganiza koyenera motsutsana ndi zofunikira zamakampani.
Wotsimikizika > 90% RNA yosawonongeka (RIN ≥ 8.0) pazovuta zamapulogalamu monga kutsatizana kwa selo imodzi.
Wothandizira Intro
Malingaliro a kampani Shanghai Lingjun Biotechnology Co., Ltd. idakhazikitsidwa mu 2016 ndipo ndi katswiri wopereka zida za biomagnetic ndi ma nucleic acid kuchotsa reagents.
Tili ndi chidziwitso chochuluka pakuchotsa nucleic acid ndi kusefera, kusefa kwabwino kwa mapuloteni, kupatukana kwa cell, chemiluminescence ndi madera ena osiyanasiyana aukadaulo.
Zogulitsa zathu zimagwiritsidwa ntchito kwambiri m'magawo ambiri, monga kuyezetsa mankhwala, kuyezetsa majini, kafukufuku wa yunivesite, kuswana chibadwa, ndi zina zotero. Sitingopereka zinthu zokha koma titha kupanganso OEM, ODM, ndi zosowa zina. Ngati muli ndi zosowa zokhudzana ndi za dna kudzipatula ndi kuchotsa, chonde omasuka kulankhula nafe [email protected].
Zolemba ndi zithunzi zonse zachokera pa intaneti. Ngati pali zovuta za kukopera, chonde titumizireni mu nthawi kuti muchotse.
Tifunseni