Hadhuudh Magnetik-cusub ee Farsamada Casriga ah waxay keentaa Nadiifinta RNA Sare, Soo noqo, iyo Waxtarka Barashada Molecular.
Shanghai, Shiinaha– [15Julaay th]– Lnjnbio (Hal qayb oo ka mid ah Shanghai Lingjun Biotechnology Co., Ltd., Ltd.), hormuudka ka ah iibiyeyaasha casriga ah ee daaweynta cilmi-baarista cilmi-baarista nolosha, waxaa sharaf u ah inay ku dhawaaqdo sii daynta Hadhuudhkeeda Magnetic ee Unugyada Xayawaanka Wadarta Qalabka Saaridda RNA. Qalabkan casriga ah waxaa loogu talagalay in lagu wanaajiyo ka saarista heerka sare ee guud ee RNA laga soo bilaabo unugyo xayawaan ah oo aad u tiro badan., siinta saynisyahannada si hufan, la isku halayn karo, iyo beddelka-saaxiibtinimo ee otomaatigga ah ee hababka ka saarista tiirarka-dhaqameedka iyo phenol-chloroform.
Kacaanka Soo saarista RNA ee Tignoolajiyada Hadhuudhka Magnetic
Xirmada ka saarida unugyada RNA ee Lnjnbio Magnetic Beads ee Isugeynta Unugyada Xayawaanka waxay shaqaaleysiisaa nidaam shaandhayn ku salaysan birlab birlabeed gaar ah kaas oo ka dhigaya qaar degdeg ah, RNA daahirsanaan sare leh oo go'doomin ah oo leh waqti-gacmo ugu yar. Adigoo ka faa'ideysanaya kimistariga la sarreeyay iyo kala-soocidda birlabeedka, xirmadani waxay meesha ka saaraysaa baahida centrifugation, shaandhaynta faakuumka, ama dareerayaasha dabiiciga ah ee waxyeelada leh, si aad ah hoos u dhac ku yimid wakhtiga wax-qabadka iyadoo si buuxda looga faa'iidaysanayo xasilloonida RNA.
(Lnjnbio Biraha Magnetic ee loogu talagalay Isku-darka Unugyada Xayawaanka ee Xirmada Soo Saaridda RNA)
Astaamaha Qarsoon & Faa'iidooyinka
Nadiifnimada RNA aan caadi ahayn & Soo noqo
Kuul birlabeedka gaarka ah ayaa si gaar ah u xidha RNA iyaga oo si sax ah uga takhalusaya wasakhowga sida borotiinnada, DNA-da genomic, iyo ka hortagga enzymatic.
Qiyaasta A260/A280 si joogto ah ≥ 1.9, samaynta qaar ka mid ah daahirnimo ku habboon codsiyada hoose sida NGS iyo qPCR.
Degdeg ah & Socodka Shaqada ee Saaxiibtinimada leh
Ka saarida dhammaystiran ugu yaraan 20– 30 daqiiqado, horumar la taaban karo oo laga sameeyay habab dhaqameed.
Ma jiro centrifugation ama tiirarka wareejinta oo loo yeedhay– isku qas kaliya, xidhid, nadiif ah, iyo elute.
Nidaamka xannibaadda lysis ee waxtarka leh ayaa dammaanad qaadaya khalkhal buuxa oo unugyada xitaa tusaaleyaal aan dhab ahayn ama dufan leh.
Automation-Diyaar u ah Shaybaadhka Waxsoosaarka Sare
Si buuxda ugu habboon robots wax ka qabashada dareeraha (tusaale., KingFisher, Biomec, Tecan) si aan kala go 'lahayn loogu dhex milmo qulqulka shaqada ee otomaatiga ah.
Ku habboon cilmi-baadhisyo waaweyn oo genomic ah, daraasad cilmi baaris xirfadeed, iyo codsiyada warshadaha.
( Electropherogram ee Lnjnbio Magnetic Beads)
Awood xidhid firfircoon: Inta badan 50 µg RNA halkii mg oo badarka ah, oo ku habboon tiro balaadhan oo ah miisaanka unugyada gelinta (10– 50 mg).
Dammaanad-qaade-RNase-free: Dhammaan walxaha si adag ayaa loo tijaabiyaa si looga fogaado xumaan RNA.
Farsamo Xoogan
1. Waxtarka Hadhuudhka Magnetik ee Sare
Qurxinta Quruxda: Qaybaha birlabeedka silica-dahaarka leh ee awoodda sare leh oo leh cabbir joogto ah (1– 3 µm) Hubi waxqabadka isku-xidhka RNA ee joogtada ah.
Awood xidhid firfircoon: Inta badan 50 µg RNA halkii mg oo kuul ah, ku rakibida tiro balaadhan oo ah miisaanka unugyada wax gelinta (10– 50 mg).
Hubinta-RNase-free: Dhammaan qaybaha si adag ayaa loo qiimeeyaa si loo joojiyo xumaanta RNA.
2. Nidaamka Buffer ee ugu sarreeya
Lysis Barrier: Si degdeg ah ayuu u kala gooyaa unugyada inta uu xasilinayo RNA, xataa jawiga qani ku ah RNase.
Dhar-dhaqidda: Si guul leh uga saar wasakhda adoon khatar gelin wax-soo-saarka RNA.
Caqabadda Illaha: Habka EDTA-hooseeya waxa uu ka dhigayaa is waafajin gaar ah oo leh qiimaynta hoose ee xasaasiga ah.
3. Xakamaynta Tayada Balaadhan
Dufcad kastaa waxay martaa baaritaanka endotoxin, Aqoonsiga DNAse/RNase, iyo qiimaynta hufnaanta ee ka soo horjeeda shuruudaha warshadaha.
La dammaanad qaaday > 90% RNA aan dhaawacmin (RIN ≥ 8.0) codsiyada dalbanaya sida isku xigxiga hal unug.
Hordhac Bixiyaha
Hal qayb oo ka mid ah Shanghai Lingjun Biotechnology Co., Ltd., Ltd. ayaa lagu aasaasay 2016 waana bixiye ku takhasusay agabka biomagnetic iyo reagents ka saarida aashitada nucleic.
Waxaan khibrad qani ah u leenahay ka saarista iyo sifaynta aashitada nucleic, sifaynta borotiinka caafimaad qabta, kala soocidda unugga, chemiluminescence iyo meelo kale oo farsamo oo kala duwan.
Alaabadayada waxaa si weyn looga isticmaalaa meelo badan, sida baaritaanka caafimaadka, baaritaanka hidda-socodka, cilmi-baaris jaamacadeed, taranta hidde, iyo wixi la mida. Ma bixinno oo kaliya alaabada laakiin sidoo kale waxaan samayn karnaa OEM, ODM, iyo baahiyo kale. Haddii aad ka qabto baahiyo la xidhiidha go'doominta dna iyo saarista, fadlan xor u noqo inaad nagala soo xidhiidho [email protected].
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