I-Innovative Magnetic Grain Modern Technology Iletha Ubumsulwa be-RNA obuphezulu, Buyela, kanye Nokuphumelela Ocwaningweni Lwengqamuzana.
Shanghai, China– [15Julayi]– I-Lnjnbio (I-Shanghai Lingjun Biotechnology Co., Ltd.), umphakeli ohamba phambili wamakhambi ocwaningo lwesayensi yempilo eyindida, kuyahlonishwa ukumemezela ukukhululwa kwe-Magnetic Grains yayo ye-Pet Cells Total RNA Removal Kit. Le sethi eyinkimbinkimbi iklanyelwe ukuthuthukisa ukuhlukaniswa kwe-RNA ephezulu kakhulu kusukela kuhlu olukhulu lwezicubu zezilwane., ukuhlinzeka ososayensi ngempumelelo, okuthembekile, kanye nezinye izindlela ezisebenziseka kalula ezisekelwe kukholomu nezindlela zokususa ze-phenol-chloroform.
I-Revolutionizing RNA Extraction nge-Magnetic Grain Technology
I-Lnjnbio Magnetic Beads for Animal Tissue Total RNA Removal Package isebenzisa uhlelo olukhethekile lokuhlunga olususelwe kubuhlalu obunobuhlalu elenza ukushesha okuthile., ukuhlukaniswa kwe-RNA okuhlanzeke kakhulu okunesikhathi esincane sokusebenza. Ngokusebenzisa ikhemistri engaphezulu kanye nokuhlukaniswa kazibuthe, le phakheji isusa isidingo se-centrifugation, ukuhlunga kwe-vacuum, noma izincibilikisi zemvelo eziyingozi, kunciphisa kakhulu isikhathi sokucubungula ngenkathi usebenzisa ngokugcwele ukuzinza kwe-RNA.
(Lnjnbio Ubuhlalu Magnetic for Animal Tissue Isamba RNA Isizinda Kit)
Izici Eziyimfihlo & Izinzuzo
Ubumsulwa be-RNA obungajwayelekile & Buyela
Ubuhlalu obukhethekile obukazibuthe bubopha i-RNA ngokuhlukile kuyilapho bususa kahle ukungcola okufana namaprotheni, i-genomic DNA, kanye nokuvimbela i-enzymatic.
Izilinganiso ze-A260/A280 njalo ≥ 1.9, ukwenza ubumsulwa obuthile bezinhlelo zokusebenza ezansi nomfula njenge-NGS ne-qPCR.
Ngokushesha & Ukugeleza komsebenzi Okusebenziseka kalula
Qedela ukususwa ngesikhathi esincane esingama-20– 30 imizuzu, ukuthuthukiswa okuphawulekayo kuzindlela zendabuko.
Akukho ukudluliswa kwe-centrifugation noma ikholomu okudingekayo– hlanganisa nje, bopha, ihlanzekile, futhi elute.
Uhlelo olusebenzayo lwe-lysis barrier luqinisekisa ukuphazamiseka okugcwele kwezicubu ngisho nezibonelo ezimahhadla noma ezinothile nge-lipid.
I-Automation-Ilungele Amalebhu Wokudlula Okuphezulu
Ifaneleka ngokuphelele ngamarobhothi abamba uketshezi (isib., InkosiFisher, I-Biomec, I-Tecan) ukufanisa okungenamthungo ekugelezeni komsebenzi okuzenzakalelayo.
Ifanele ucwaningo olukhulu lwe-genomic, ucwaningo lochwepheshe, kanye nezicelo zezimboni.
( I-Electropherogram ye-Lnjnbio Magnetic Beads)
Amandla Okubopha Amandla: Kangango ba 50 µg i-RNA nge-mg yokusanhlamvu, ifanela uhla olukhulu lwesisindo samaseli okokufaka (10– 50 mg).
Isiqinisekiso samahhala se-RNase: Zonke izici zivivinywa ngokuqinile ukuze kugwenywe ukuwohloka kwe-RNA.
Ukugcizelela Kwezobuchwepheshe
1. I-Superior Magnetic Grain Efficiency
Ubuhlalu Make-up: Izinhlayiya ezinozibuthe ezinomthamo omkhulu we-silica ezinosayizi ongashintshi (1– 3 µm) qinisekisa ukusebenza okungaguquki kokubopha kwe-RNA.
Amandla Okubopha Amandla: Kangango ba 50 µg i-RNA nge-mg yobuhlalu, ukufaka uhla olukhulu lwezisindo zamaseli okokufaka (10– 50 mg).
Isiqinisekiso Samahhala se-RNase: Zonke izingxenye zihlolwa ngokuqinile ukuze kumiswe ukuwohloka kwe-RNA.
2. Isistimu ye-Buffer ephezulu
I-Lysis Barrier: Iphazamisa ngokushesha amaseli ngenkathi iqinisa i-RNA, ngisho nasezindaweni ezicebile ze-RNase.
Ama-Laundry Buffers: Ukuqeda ngempumelelo ukungcola ngaphandle kokubeka engcupheni isivuno se-RNA.
I-Elution Barrier: Ukwakhiwa kwe-EDTA ephansi kwenza ukuhambisana okuthile nezivivinyo ezibucayi zomfula.
3. Ukulawulwa Kwekhwalithi Okubanzi
Iqoqo ngalinye lidlula ekuhlolweni kwe-endotoxin, Ukuqashelwa kwe-DNase/RNase, kanye nokulinganisa ngendlela efanele uma kuqhathaniswa nemibandela yezimboni.
Kuqinisekisiwe > 90% i-RNA engalimele (I-RIN ≥ 8.0) kuzinhlelo zokusebenza ezifunayo njengokulandelana kweseli elilodwa.
Isingeniso Somhlinzeki
I-Shanghai Lingjun Biotechnology Co., Ltd. yasungulwa ngo 2016 futhi ungumhlinzeki onguchwepheshe wezinto ezisetshenziswayo ze-biomagnetic kanye nama-nucleic acid okususa ama-reagents.
Sinolwazi olucebile ekususeni nasekuhlungeni i-nucleic acid, ukuhlunga amaprotheni okunempilo, ukuhlukaniswa kweseli, i-chemiluminescence nezinye izindawo ezihlukahlukene zobuchwepheshe.
Imikhiqizo yethu isetshenziswa kabanzi emikhakheni eminingi, njengokuhlolwa kwezokwelapha, ukuhlolwa kofuzo, ucwaningo lwasenyuvesi, ukuzalanisa izakhi zofuzo, njalo njalo. Asihlinzeki ngemikhiqizo kuphela kodwa futhi singenza i-OEM, I-ODM, nezinye izidingo. Uma unezidingo ezihlobene mayelana ukuhlukaniswa kwe-dna nokukhipha, sicela ukhululeke ukuxhumana nathi [email protected].
Zonke izindatshana nezithombe zivela ku-inthanethi. Uma kukhona izinkinga ze-copyright, sicela usithinte kusenesikhathi ukuze ususe.
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