Fa'aonaponei Fa'atekonolosi Fa'aonaponei Fa'amagnet E Tu'uina atu le Mama RNA Sili, Toe fo'i, ma le Lelei mo Suʻesuʻega Molecular.
Shanghai, Saina– [15ia Iulai]– Lnjnbio (Shanghai Lingjun Biotechnology Co., Ltd.), ose ta'ita'i fa'atau oloa su'esu'ega su'esu'ega fa'asaienisi olaga, ua fa'amamaluina e fa'ailoa le tu'uina atu o ana Magnetic Grains mo Pet Cells Total RNA Removal Kit. O lenei seti faʻapitoa ua mamanuina e faʻaleleia ai le faʻaesea o le maualuga o le RNA atoa mai le tele o aano o manu., tu'uina atu i saienitisi se lelei, faatuatuaina, ma isi faiga fa'aautometi e fa'atatau ile koluma masani ma auala e aveese ai le phenol-chloroform.
Revolutionizing RNA Extraction with Magnetic Grain Technology
Ole Lnjnbio Magnetic Beads for Animal Tissue Total RNA Removal Package o lo'o fa'aogaina ai se faiga fa'amama fa'avae fa'amaneta fa'avae e fa'avavevave ai., maualuga-mama RNA fa'aesea ma itiiti lima-i luga o le taimi. E ala i le fa'aogaina o le kemisi maualuga ma le vavae'ese maneta, e aveesea e lenei afifi le manaomia mo centrifugation, fa'amama fa'amama, po'o so'o fa'alenatura leaga, matua fa'aitiitia taimi fa'agaioiga a'o fa'aoga lelei le mautu RNA.
(Lnjnbio Magnetic Beads mo Tissue Manu Aofa'i RNA Extraction Kit)
Mea lilo & Tulaga lelei
Fa'apitoa RNA mama & Toe fo'i
O lo'o fa'amageta fa'apitoa e fusia fa'apitoa ai le RNA a'o fa'ate'a lelei mea filogia e pei o polotini, genomic DNA, ma puipuiga enzymatic.
A260/A280 fua faatatau i taimi uma ≥ 1.9, faia fa'apitoa mama atoatoa mo fa'aoga i lalo e pei ole NGS ma le qPCR.
Vave & Fa'aoga-Faauoga Galulue
Aveese atoa ile 20– 30 minute, ose fa'aleleia tele nai lo faiga masani.
Leai se centrifugation po'o se koluma fesiitaiga e mana'omia– na'o le fefiloi, fusifusia, mama, ma elute.
O le fa'aogaina lelei o le lysis barrier system e fa'amautinoa ai le fa'alavelaveina o le tino e o'o lava i fa'ata'ita'iga lapo'a po'o le lipid-oaoa.
Otometi-Sauni mo Labs-Throughput maualuga
E fetaui lelei ma robots e taulimaina vai (e.g., KingFisher, Biomec, Tecan) mo le fa'aogaina lelei i totonu o galuega fa'a otometi.
E fetaui mo suʻesuʻega genomic tetele, su'esu'ega fa'apolofesa, ma talosaga tau alamanuia.
( Electropherogram o Lnjnbio Magnetic Beads)
Malosiaga Fa'amauina: E pei o 50 µg RNA ile mg o fatu, fetaui ma le tele o fua o sela fa'aoga (10– 50 mg).
RNase-Free Guarantee: O elemene uma e faʻataʻitaʻiina faʻamalosi e aloese mai le faʻaleagaina o le RNA.
Fa'amamafaina Fa'apitoa
1. Fa'amatagofieina o Saito maneta
Make-up Bead: O vaega maneta e fa'apipi'iina le silica maualuga ma le lapopo'a tutusa (1– 3 µm) faʻamautinoa le faʻatinoina o le faʻaogaina o le RNA.
Malosiaga Fa'amauina: E pei o 50 µg RNA ile mg o pulu, fa'apipi'i le tele o fua o sela fa'aoga (10– 50 mg).
RNase-Saoloto Fa'amautinoaga: O vaega uma e matua'i su'esu'eina e taofi le fa'aleagaina o le RNA.
2. Fa'atelega Faiga Fa'apolopolo
Lysis Papuipui: Faʻalavelave vave sela aʻo faʻamautu le RNA, e oʻo lava i le RNase-mauoa atemosifia.
Taofi Taofi: Ia manuia le aveesea o mea eleelea e aunoa ma le afaina o le RNA.
Elution Pa: Ole fa'atulagaina ole Low-EDTA e maua ai le fetaui lelei ma su'ega ma'ale'ale i lalo.
3. Pulea lelei tele
O vaega ta'itasi e alu ile su'ega endotoxin, DNase/RNase faailoaina, ma fa'atusatusaga lelei e fa'atatau i tulaga tau alamanuia.
Fa'amaonia > 90% RNA e leʻi faʻaleagaina (RIN ≥ 8.0) mo talosaga mana'omia e pei o le fa'asologa o sela e tasi.
Fa'atomuaga a le Fa'atonu
Shanghai Lingjun Biotechnology Co., Ltd. sa faatuina i 2016 ma ose fa'apitoa e tu'uina atu mea fa'aola ma mea e fa'ate'aina ai le nucleic acid.
E tele a matou poto masani i le aveesea ma le faamamaina o le nucleic acid, filtration polotini maloloina, vavaeeseina o sela, chemiluminescence ma isi vaega faʻapitoa.
O a matou oloa e faʻaaogaina lautele i le tele o fanua, pei ole su'ega faafoma'i, su'ega fa'a kenera, su'esu'ega iunivesite, fa'atupuina o kenera, ma faapena atu ai lava. Matou te le gata ina tuʻuina atu oloa ae mafai foi ona faʻatinoina OEM, ODM, ma isi manaoga. Afai ei ai ni ou mana'oga fa'atatau e uiga i DNA tu'ufua ma le fa'amama, fa'amolemole lagona le saoloto e fa'afeso'ota'i i matou i le [email protected].
O tala uma ma ata e mai le Initaneti. Afai e iai ni fa'afitauli o le puletaofia, faamolemole faafesootai matou i le taimi e tape ai.
Fesili mai ia i matou